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背景:研究证实心房钠尿肽受体在大鼠胃的不同分区其分布密度不尽相同,其可抑制大鼠、豚鼠和人胃平滑肌的自发性收缩。目的:探讨心房钠尿肽合成细胞在大鼠胃黏膜的超微结构定位及与微血管密度的相关关系。设计:单一样本观察。单位:承德医学院附属医院。材料:清洁级成年雄性Wistar大鼠18只,体质量300～350g。方法:实验于2004-10/2007-07在承德医学院中药研究所免疫学实验室完成。用包埋后免疫胶体金标记的电镜技术对大鼠胃黏膜心房钠尿肽-合成细胞进行超微结构定位,用组织化学藏红花-甲苯胺蓝染色对心房钠尿肽-合成细胞进行标记,同时用单宁酸-氧化铁和扫描电镜技术对大鼠胃黏膜的微血管进行观察。主要观察指标:①心房钠尿肽合成细胞的在大鼠胃内的超微结构定位。②微血管在大鼠胃黏膜的分布特点。③心房钠尿肽合成细胞的在大鼠胃黏膜的分布。④心房钠尿肽合成细胞与胃黏膜中微血管分布的相关关系。结果:①大鼠胃黏膜心房钠尿肽-合成细胞超微结构定位:证实心房钠尿肽-合成细胞为胃黏膜的肠嗜铬细胞。②大鼠胃黏膜的微血管观察结果:在胃黏膜的不同切面上微血管在腺体间弯曲走行在不同的切面上显示出三维结构,在胃黏膜的基底部微血管的密度最大。③心房钠尿肽合成细胞的在大鼠胃黏膜的分布:证实肠嗜铬细胞是胃黏膜的唯一嗜铬细胞,胞浆内有棕色颗粒,而在黏膜下和肌层中没有这种细胞分布,肠嗜铬细胞在不同的分布区域其分布密度不相同,分布密度最大在贲门区,其次是幽门区和胃底区,其分布密度排列顺序依次是:贲门区>幽门区>胃底区。④心房钠尿肽合成细胞与胃黏膜中微血管分布的相关关系:在大鼠胃贲门区两者呈正相关(r=0.53,P<0.05,n=18);而在幽门区没有相关关系(r=0.38,P>0.05,n=18);在胃底区亦没有相关关系(r=-0.29,P>0.05,n=18)。结论:心房钠尿肽-合成细胞是胃黏膜的肠嗜铬细胞,心房钠尿肽-合成细胞和微血管在大鼠胃贲门区二者呈正相关,而在幽门区和胃底区没有相关性。
BACKGROUND: Studies have demonstrated that atrial natriuretic peptide receptors vary in their distributional density in different compartments of the rat stomach, which can inhibit spontaneous contractions in rat, guinea pig and human gastric smooth muscle. Objective: To investigate the ultrastructural localization of atrial natriuretic peptide-producing cells in rat gastric mucosa and its relationship with microvessel density. Design: Single sample observation. Unit: Chengde Medical College Hospital. MATERIALS: Eighteen adult male Wistar rats of clean grade with body weight of 300 ~ 350g. Methods: The experiment was performed at Immunology Laboratory of Chinese Materia Medica Institute, Chengde Medical College from October 2004 to July 2007. The ultrastructure of gastric atrial natriuretic peptide - synthetic cells in gastric mucosa of rats was observed by electron microscopy after immunogold labeling. The atrial natriuretic peptide - synthetic cells were stained with histochemical saffron-toluidine blue staining Microencapsulation of gastric mucosa in rats was observed with tannic acid-iron oxide and scanning electron microscopy. MAIN OUTCOME MEASURES: ①Ultrastructural localization of atrial natriuretic peptide in rat stomach. ② microvascular in gastric mucosa of the distribution characteristics. ③ atrial natriuretic peptide synthesis in rat gastric mucosa distribution. ④ atrial natriuretic peptide synthesis cells and gastric mucosa in the relationship between microvascular distribution. Results: ① The ultrastructure of atrial natriuretic peptide - synthetic cells in gastric mucosa of rats: Confirmation of atrial natriuretic peptide - synthetic cells as enterochromaffin cells of gastric mucosa. (2) The results of microvessel observation in gastric mucosa of rats: The microvessels in the different sections of gastric mucosa showed a three-dimensional structure in different sections, and the microvessel density was the highest in the basal part of gastric mucosa. (3) Distribution of atrial natriuretic peptide in rat gastric mucosa: It was confirmed that enterochromaffin cells were the only chromaffin cells in gastric mucosa with brown granules in the cytoplasm, but not in the submucosa and myometrium . The distribution density of enterochromaffin cells in different distribution areas is different, the distribution density is the largest in the cardia area, followed by the pyloric area and the fundic fundus area. The order of distribution density of enterochromaffin cells is: cardia area> pylorus area> fundus area. ④Atrial natriuretic peptide synthesis and gastric mucosa in the distribution of microvessels in the gastric cardia area of the two was positively correlated (r = 0.53, P <0.05, n = 18); in the pylorus area there is no correlation (r = 0.38, P> 0.05, n = 18). There was no correlation in the fundic region (r = -0.29, P> 0.05, n = 18). CONCLUSION: Atrial natriuretic peptide-synthesized cells are enterochromaffin cells of gastric mucosa. Atrial natriuretic peptide-synthesized cells and microvessels are positively correlated with gastric cardia in rats, but not in pylorus and gastric fundus.