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目的建立SD大鼠C6胶质瘤模型并对其病理特征及MRI进行观察。方法50只SD大鼠随机分成5组,每组10只,C6胶质瘤细胞悬液立体定向接种于大鼠的右侧尾状核,接种后观察大鼠的生活状态、生存期;分别于接种后不同时段进行MRI观察肿瘤生长特性及肿瘤体积的测量;取不同时段组大鼠脑标本行脑组织HE染色、透射电镜(transmissionelectronmicroscope,TEM)、脑组织含水量测量(与10只正常大鼠对照)、胶质纤维酸性蛋白(GFAP)免疫组化检查。结果立体定向颅内接种成功率97.5%,未见远处及颅外转移,肿瘤在一定时期内生长较快,脑水肿随肿瘤的生长明显加重,生存期观察组中7只荷瘤鼠死亡,3只肿瘤自发部分消退。结论立体定向建立大鼠C6胶质瘤模型成功率高,接种后颅内肿瘤呈浸润性生长,与人脑胶质瘤具有相似性,由于生存期观察组中有部分荷瘤鼠出现肿瘤自发部分消退,故应用该模型评价治疗效果时应慎重;T1WI增强扫描可清晰显示肿瘤影像,且能更早发现肿瘤;MRI联合病理可较好反映肿瘤生长方式及发展过程。
OBJECTIVE: To establish a rat C6 glioma model and observe its pathological features and MRI. Methods Fifty Sprague-Dawley rats were randomly divided into five groups with 10 rats in each group. C6 glioma cell suspension was stereotactically inoculated to right caudate nucleus of rats. After inoculation, the living conditions and survival of rats were observed. Tumor growth characteristics and tumor volume were measured by MRI at different time points after inoculation. HE staining, transmission electron microscope (TEM), brain water content measurement (compared with 10 normal rats Control), glial fibrillary acidic protein (GFAP) immunohistochemistry. Results Stereotactic intracranial vaccination success rate of 97.5%, no distant and extracranial metastasis, the tumor grew rapidly within a certain period of time, brain edema significantly increased with the growth of the tumor, the survival of the observation group of 7 tumor-bearing mice died, Three tumors spontaneously subsided. Conclusion The stereotactic model of rat C6 glioma has a high success rate. After inoculation, the intracranial tumors showed invasive growth with similarities with human glioma. Since some tumor-bearing mice in the observation group showed spontaneous tumor Therefore, the model should be used with caution when evaluating the therapeutic effect. T1WI enhanced scan can clearly show the tumor image and detect the tumor earlier. The MRI combined pathology can better reflect the tumor growth pattern and development process.