论文部分内容阅读
目的:建立复方肝素钠乳膏抗Ⅱα因子活性的测定方法。方法:采用微量生色底物法,按生物检定统计法中量反应平行线原理4×4法完全随机实验设计,测定复方肝素钠乳膏抗Ⅱα因子活性。结果:肝素钠抗Ⅱα因子活性在0.005 04~0.021 IU·ml~(-1)范围内线性关系良好(r=0.992),平均回收率为101.6%(RSD=2.76%,n=9)。结论:本法准确、可靠、重复性好,可有效控制复方肝素钠乳膏质量。
Objective: To establish a method for determination of anti-Ⅱα activity of compound heparin sodium cream. Methods: The method of trace chromogenic substrate method was used to determine the anti-Ⅱα activity of compound heparin sodium cream by the randomized experimental design of 4 × 4 method. Results: The activity of heparin Ⅱ a had a good linear relationship (r = 0.992) in the range of 0.005 04 ~ 0.021 IU · ml -1 with an average recovery of 101.6% (RSD = 2.76%, n = 9). Conclusion: This method is accurate, reliable and reproducible, which can effectively control the quality of compound heparin sodium cream.