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目的建立测定蜂蜜中土霉素和盐酸金霉素含量的方法。方法采用紫外光度法测定土霉素和盐酸金霉素的含量。结果在甲醇溶液中,土霉素和盐酸金霉素的最大吸收波长分别在364nm和319nm处,在0.56～6.72g/mL和1.03～20.60g/mL浓度范围内有很好的线性关系,回归方程和相关系数分别为Y=0.0254X0.00644,r=0.99355和Y=0.02391+0.02756X,r=0.99493。结论该方法操作简单,重现性好,可准确用于蜂蜜样品中土霉素和盐酸金霉素含量的测定,结果满意。
Objective To establish a method for the determination of oxytetracycline hydrochloride and chlortetracycline hydrochloride in honey. Methods The contents of oxytetracycline and chlortetracycline hydrochloride were determined by ultraviolet spectrophotometry. Results In methanol solution, the maximum absorption wavelength of oxytetracycline and chlortetracycline hydrochloride had good linearity at 364nm and 319nm, respectively, within the range of 0.56 ~ 6.72g / mL and 1.03 ~ 20.60g / mL. The regression equation The equations and correlation coefficients were Y = 0.0254X0.00644, r = 0.99355 and Y = 0.02391 + 0.02756X, r = 0.99493, respectively. Conclusion The method is simple, reproducible and can be used to determine oxytetracycline hydrochloride and chlortetracycline hydrochloride in honey samples with satisfactory results.