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目的探讨松花粉总甾醇对丙酸睾酮(TP)诱导大鼠前列腺增生的抑制作用及机制。方法将60只SD大鼠随机分为对照组、模型组、阳性对照组和松花粉甾醇低、中、高剂量组,其中对照组大鼠正常喂饲,其他组腹腔注射丙酸睾酮注射液5 mg/kg·d。低、中、高剂量组同时灌胃不同剂量松花粉甾醇(10 mg/kg·d、20 mg/kg·d、50 mg/kg·d),阳性对照组给予β-谷甾醇(20 mg/kg·d)连续28 d。28d后,摘眼球取血处死动物,取前列腺组织、睾丸组织并称重;前列腺组织石蜡切片HE染色观察组织形态,用ELISA测定大鼠血清中前列腺特异性抗原(PSA)、睾酮(T)和双氢睾酮(DHT)的水平;用高效液相色谱(HPLC)法测定5α-还原酶活性。结果松花粉甾醇中、高剂量组大鼠前列腺湿重及指数相对模型组显著降低(P值分别为:0.014,0.047;0.000,0.002);显微镜下观察,松花粉总甾醇低、中、高剂量组均可缓解由丙酸睾酮所致的大鼠前列腺增生;与模型组比较,松花粉甾醇中剂量组和高剂量组大鼠血清DHT和PAS显著降低(P值分别为:0.038,0.019;0.008,0.002);与模型组比较,松花粉甾醇中剂量组和高剂量组大鼠前列腺5α-还原酶活性显著降低,差异有统计学意义(P值分别为:0.016,0.000)。结论松花粉甾醇具有良好的抑制前列腺增生的作用,其机制通过抑制5α-还原酶降低大鼠体内双氢睾酮的水平来抑制前列腺增生。
Objective To investigate the inhibitory effect and mechanism of pine pollen total sterol on prostate hyperplasia induced by testosterone propionate (TP) in rats. Methods Sixty Sprague-Dawley rats were randomly divided into control group, model group, positive control group and low, medium and high dosage groups of pineal sterol. The control group rats were fed with normal and the other groups were intraperitoneally injected with testosterone propionate injection 5 mg / kg · d. The low, medium and high dose groups were given gavage at different dosages (10 mg / kg · d, 20 mg / kg · d, 50 mg / kg · d) and the positive control group received β-sitosterol kg · d) for 28 days. Twenty-eight days later, the animals were sacrificed and the animals were sacrificed. Prostate and testicular tissues were obtained and weighed. The histopathology of the prostate tissues was observed by HE staining. The levels of prostate-specific antigen (PSA) and testosterone (T) Dihydrotestosterone (DHT) levels; 5α-reductase activity was determined by high performance liquid chromatography (HPLC). Results Compared with model group, the wet weight and index of prostate in the medium and high dose of the rosette sterol group were significantly decreased (P = 0.014,0.047; 0.000,0.002, respectively); under the microscope, the total, Group could relieve benign prostatic hyperplasia in rats induced by testosterone propionate. Compared with model group, DHT and PAS in middle dose and high dose of pineal sterol group were significantly decreased (P = 0.038, 0.019, 0.008 , 0.002). Compared with the model group, the activity of 5α-reductase in the prostate of middle-dose and high-dose groups decreased significantly (P = 0.016,0.000, respectively). CONCLUSIONS: Polysterol has a good inhibitory effect on benign prostatic hyperplasia. Its mechanism inhibits benign prostatic hyperplasia by inhibiting the decrease of dihydrotestosterone in rats by 5α-reductase.