论文部分内容阅读
采用激光扫描共聚焦显微镜观察受不同参数高功率微波辐照后的大鼠精子细胞内Ca2+的变化,同时观察了辐照后大鼠的生殖细胞微核率、畸形率、精子数量、存活率和顶体酶反应的变化。实验表明:此条件下的高功率微波辐照对雄性大鼠的生殖细胞微核率和畸形率没有明显的损伤效应,但4×105次脉冲的高功率微波辐照对大鼠的顶体酶反应有较明显的抑制作用,可能通过改变细胞内第二信使的Ca2+浓度来影响其生殖细胞功能。
Laser scanning confocal microscopy was used to observe the changes of intracellular Ca2 + in spermatids of rats exposed to high power microwaves with different parameters. Meanwhile, the micronucleus rate, malformation rate, sperm number and survival rate of irradiated gerbils were observed Changes in acrosin reaction. Experiments show that: under this condition, high power microwave irradiation has no obvious damaging effect on the germ cell micronucleus rate and malformation rate in male rats. However, 4 × 105 pulses of high power microwave irradiation on acrosin The reaction has a more pronounced inhibitory effect, may affect the germ cell function by changing the second messenger Ca2 + concentration in the cell.