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目的研究子痫前期患者外周血单个核细胞分泌Th1/Th2细胞因子功能变化。方法子痫前期患者29例,正常妊娠妇女15例,分离外周血单个核细胞,体外培养72小时,ELISA方法检测培养上清液中Th1型细胞因子IL-2、IFN-γ及Th2型细胞因子IL-4、IL-10水平。结果子痫前期组PBMC培养上清液中,Th1型细胞因子IL-2、IFN-γ水平(259.4±90.5)pg/mL,(452.0±122.6)pg/mL与正常妊娠组(140.3±73.2)pg/mL,(307.5±106.4)pg/mL相比,显著升高,分别为P<0.01,P<0.05;Th2型细胞因子IL-4、IL-10水平(28.8±7.7)pg/mL,(178.9±98.6)pg/mL与正常妊娠组(41.9±11.4)pg/mL,(316.1±284.6)pg/mL相比,显著下降,分别为P<0.01,P<0.05。结论子痫前期患者外周血单个核细胞分泌Th1型细胞因子功能增强,分泌Th2型细胞因子功能减弱。
Objective To study the functional changes of Th1 / Th2 cytokines secreted by peripheral blood mononuclear cells in patients with preeclampsia. Methods Twenty-nine patients with preeclampsia and 15 normal pregnant women were divided into peripheral blood mononuclear cells and cultured in vitro for 72 hours. Th1 cytokines IL-2, IFN-γ and Th2 cytokines IL-4, IL-10 levels. Results Compared with the normal pregnancy group (140.3 ± 73.2), the levels of Th1 cytokines IL-2 and IFN-γ in PBMC culture supernatant of preeclampsia group (259.4 ± 90.5) pg / mL and (452.0 ± 122.6) pg / Pg / mL, (307.5 ± 106.4) pg / mL, P <0.01 and P <0.05 respectively; Th2 cytokines IL-4 and IL-10 levels were 28.8 ± 7.7 pg / (178.9 ± 98.6) pg / mL was significantly lower than that of the normal pregnant group (41.9 ± 11.4 pg / mL, 316.1 ± 284.6 pg / mL, P <0.01, P <0.05 respectively. Conclusions Peripheral blood mononuclear cells from patients with preeclampsia have enhanced secretion of Th1 cytokines and weakened secretion of Th2 cytokines.