论文部分内容阅读
最近Dzick和Necker(1983)曾发现,当用淋巴细胞亚群的单抗作荧光激活细胞分离器(FACS)分析时,4℃保存全血过夜改变了淋巴细胞亚群的比例;而室温保存过夜时,产生的结果和新鲜淋巴细胞制品相同.特别是Leu3(T_H)降低,导致辅助/抑制之间的比例降低.作者把这种现象叫做冷冻获得性免疫缺陷综合征(RAI=DS).Shield等(1983)、Weiblen等(1984)也报告了同样的结果.与这种现象相反,作者们从未观察到4℃保存全血过夜会对淋巴细胞亚群有影响.这就促使作者去研究这种差异.
Recently Dzick and Necker (1983) found that preserving whole blood overnight at 4 ° C altered the proportion of lymphocyte subsets when assayed by fluorescence-activated cell sorter (FACS) with lymphocyte subpopulations; overnight at room temperature Results in the same results as fresh lymphocytes, especially with a decrease in Leu3 (T_H) leading to a decrease in the ratio between adjuvant / inhibition, a phenomenon the authors call Frozen Acquired Immune Deficiency Syndrome (RAI = DS) Similar results were also reported by Weiblen et al. (1984) and Weiblen et al. (1984). Contrary to this phenomenon, the authors have never observed that overnight preservation of whole blood at 4 ° C has an effect on lymphocyte subsets, which prompted the authors to study This difference.