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目的 探讨经脂质体介导转染血管内皮生长因子 (VEGF) 12 1基因的大鼠胚胎神经干细胞体内外基因表达的特点。方法 脂质体介导法将VEGF12 1基因转染到大鼠神经干细胞中。经逆转录 聚合酶链反应 (RT PCR)及免疫荧光染色检测转基因神经干细胞及其分化细胞的基因表达情况。用立体定向法将BrdU标记的转基因神经移植到大鼠大脑中动脉梗死模型的纹状体缺血半暗区 ,免疫荧光染色观察移植后 1周转基因神经干细胞在脑内的基因表达情况。结果 转基因神经干细胞及其分化的子代细胞均有VEGF12 1的表达并持续 2周左右。转基因神经干细胞移植后 1周在宿主脑内迁徙并表达VEGF基因产物。结论 经脂质体介导转染VEGF12 1基因的大鼠胚胎神经干细胞在体内外均有良好的基因表达
Objective To investigate the gene expression in vitro and in vivo of rat embryonic neural stem cells transfected with vascular endothelial growth factor (VEGF) 12 1 by liposome. Methods VEGF12 1 gene was transfected into rat neural stem cells by liposome-mediated method. The gene expression of transgenic NSCs and their differentiated cells was detected by reverse transcriptase polymerase chain reaction (RT PCR) and immunofluorescence staining. BrdU-labeled transgenic neurons were transplanted into the ischemic penumbra of rat middle cerebral artery occlusion model by stereotactic method. The expression of genetically modified neural stem cells in the brain was observed by immunofluorescence staining 1 week after transplantation. Results The expression of VEGF12 1 in transgenic NSCs and their differentiated progeny cells was about 2 weeks. One week after transfection, neural stem cells migrate in the host brain and express VEGF gene products. Conclusion The rat embryonic neural stem cells transfected with VEGF12 1 by liposomes have good gene expression in vitro and in vivo