目的:探索美洲商陆组培快速繁殖技术。方法:以无菌苗作外植体。结果:初代培养的较粗壮茎段为快速繁殖的最佳外植体,初代培养基为MS+NAA0.2 mg/L+6-BA1.0 mg/L;丛生芽最佳增殖培养基为MS+NAA0.2mg/L+6-BA2.0 mg/L,最适生根培养基为1/2MS+NAA0.4 mg/L。结论:采用美洲商陆无菌苗茎段诱导丛生芽可提高繁殖系数,为大量种植提供种苗。 Objective: To explore the rapid propagation technology of tissue culture of Pokeweed. Methods: Aseptic shoots were used as explants. Results: The thicker stem segments of primary culture were the best explants for rapid propagation. The primary culture medium was MS + NAA 0.2 mg / L and 6-BA 1.0 mg / L. The best proliferation medium for cluster buds was MS + NAA 0.2 mg / L + 6-BA 2.0 mg / L, and the best rooting medium was 1 / 2MS + NAA 0.4 mg / L. CONCLUSION: The use of pusillus thuringiensis in the stem of aseptic seedlings of Pokeweed can increase the breeding coefficient and provide seedlings for mass planting.