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目的：通过构建人扁桃体淋巴细胞cDNA质粒重组体，为深入研究淋巴细胞的生物学功能奠定实验基础。方法：采用DNA重组技术，以人新鲜扁桃体淋巴细胞为实验材料从中提取mRNA，以mRNA为模板合成cDNA，然后与质粒pSPORTI进行定向连接并转入到E．coliJM109中进行分子克隆。结果：阳性克隆占90％，阴性克隆占10％；转化率为1．35×104克隆／微开连接体系；插入片段长度为0．5～2．3Kb，平均1．3Kb。结论：人扁桃体淋巴细胞cDNA质粒重组体构建成功。
OBJECTIVE: To establish an experimental basis for the further study of the biological function of lymphocytes by constructing a recombinant plasmid of human tonsil lymphocyte cDNA plasmids. Methods: DNA was extracted from human fresh tonsillar lymphocytes by DNA recombination technique. CDNA was synthesized using mRNA as a template and then ligated with plasmid pSPORTI. coliJM109 in molecular cloning. Results: Positive clones accounted for 90% and negative clones accounted for 10%. The transformation rate was 1.35 × 104 clones / micro open junction system. The length of inserted fragment was 0.5 ~ 2.3Kb with an average of 1.3Kb. Conclusion: The human tonsil lymphocyte cDNA plasmid recombinant was successfully constructed.