论文部分内容阅读
肿瘤细胞表面的粘附分子在恶性肿瘤浸润转移过程中起着重要作用[1]。CD44是粘附分子家族成员之一,可存在于多种恶性肿瘤中,其多种拼接变异体与肿瘤转移、复发有明显关系[2]。研究表明,CD44拼接变异体（CD44splice variants,CD44v）的表达,特别是CD44v6的表达与胃癌浸润、转移、复发关系密切[3]。我们采用流式细胞术检测CD44v6在胃癌中的表达特征及其与胃癌临床病理特征的关系,探讨CD44v6表达在判断胃癌浸润、转移及预后中的意义。 一、实验材料 1.人胃癌标本取自1999年1月至1999年8月在南京医科大学附属南京第一医院外科手术切除经病理组织学证实的胃癌患者,共36例。男性22例,女性14例;年龄30～79岁,<60岁15例,平均年龄49.9岁,≥60岁21例,平均
Tumor cell surface adhesion molecules play an important role in the invasion and metastasis of malignant tumors [1]. CD44 is a member of the adhesion molecule family can exist in a variety of malignant tumors, a variety of splicing variants and tumor metastasis, recurrence has a clear relationship [2]. Studies have shown that CD44splice variants (CD44v) expression, especially CD44v6 expression and gastric cancer invasion, metastasis, relapse are closely related [3]. We used flow cytometry to detect the expression of CD44v6 in gastric cancer and its relationship with the clinicopathological features of gastric cancer and explore the significance of CD44v6 expression in the diagnosis of gastric cancer invasion, metastasis and prognosis. First, the experimental material 1. Human gastric cancer specimens from January 1999 to August 1999 in Nanjing Medical University Affiliated Hospital of Nanjing Surgery resected histopathologically confirmed gastric cancer patients, a total of 36 cases. 22 males and 14 females; aged 30 to 79 years old, <60 years old in 15 cases, the average age of 49.9 years, ≥ 60 years old in 21 cases, the average