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目的：探讨胃癌发生发展不同阶段癌基因表达特点并予以定量分析，对阐明胃癌发生发展机理及早期诊断胃癌具有重要意义。方法：应用ras基因产物共有多肽序列单克隆抗体PAP免疫组化对照研究了13例慢性萎缩性胃炎伴肠化生（大肠型），32例胃窦癌及15例正常胃窦粘膜ras基因表达特点。结果：慢性萎缩性胃炎伴肠化生粘膜ras基因产物免疫反应阳性细胞形态较规则，呈卵圆形或低柱状，免疫反应强度及阳性细胞数量均较胃癌增强（多）（P均＜0．05）阳性细胞成片分布肠化粘膜下部及基底部，不穿越基底层；胃癌中免疫反应阳性细胞形态不规则，核分裂相明显，成团或散在分布，免疫反应强弱不等，呈异质性表达。正常胃窦粘膜可见谈棕黄色单个分布的ras产物阳性细胞。结论：ras基因在胃癌前病变慢性萎缩性胃炎伴肠化生中已激活，对ras产物精确定量分析有助于胃癌前病变的诊断。
Objective: To investigate the expression characteristics of oncogenes at different stages of gastric carcinogenesis and to quantitatively analyze them, which is of great significance for elucidating the mechanism of gastric cancer development and early diagnosis of gastric cancer. METHODS: The expression of ras gene in 13 cases of chronic atrophic gastritis with intestinal metaplasia (large intestine type), 32 cases of gastric sinus cancer and 15 cases of normal gastric antrum was studied by immunohistochemistry using the monoclonal antibody PAP of the consensus polypeptide sequence of ras gene product. . RESULTS: The immunoreactive positive cells of chronic atrophic gastritis with intestinal metaplasia ras gene products were more regular, oval or low-column, and the immune response intensity and number of positive cells were significantly (p < .0) higher than that of gastric cancer. 05) The positive cells were distributed into the submucosa and the basement of the intestine and did not cross the basal layer; the immunoreactive positive cells in the gastric cancer were irregular in morphology, with obvious mitotic figures, clustered or scattered distribution, and the immune response varied in intensity. Sexual expression. Normal antral mucosa can be seen in brownish-yellow distributed ras-positive cells. Conclusion: The ras gene has been activated in gastric precancerous chronic atrophic gastritis with intestinal metaplasia. Accurate quantitative analysis of ras products is helpful for the diagnosis of precancerous lesions.