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近年来测定体液中心得安及其主要代谢物4-羟基心得安最成功的方法是带UV吸收或荧光检测器的HPLC法,其主要缺点是必须对体液样品作净化处理.本法用预柱作净化处理,直接注入血浆样品,优点是快速、准确、不需净化分离、样品损耗低,并可自动化.预柱长2cm,id 2.1mm.内装30μm C_?微球,柱温60℃.注入样品后,预柱以0.1mol/L的磷酸液洗脱,流速为0.1ml/min,1min后排除预柱的污染废液,而心得安和4-羟基心得安在预柱上,转换
In recent years, the most successful method to determine the safety of body fluid center and its main metabolites 4-hydroxy propranolol is HPLC with UV absorption or fluorescence detector, the main disadvantage is that the body fluid samples must be purified. For purification, direct injection of plasma samples, the advantage is fast, accurate, without purification separation, sample loss and automation. Pre-column length 2cm, id 2.1mm. Built-in 30μm C_? After the sample, the pre-column was eluted with 0.1mol / L phosphoric acid solution, the flow rate was 0.1ml / min, 1min after the exclusion of the pre-column of the waste liquid, and propranolol and 4-hydroxy security in the pre-column conversion