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目的 :研究中国人载脂蛋白E(ApoE)基因多态性及血管紧张素转换酶 (ACE)基因插入 /缺失 (I/D)多态性与冠心病的关联。方法 :分别用PCR -RFLP及PCR技术检测了 12 9例冠心病患者及 90例健康人ApoE ,ACE基因型。结果 :冠心病组ε3 / 4基因型及ε4等位基因频率显著高于对照组 ,ε3 / 3 基因型及ε3 等位基因频率则显著低于对照组 ;ACE各基因型及等位基因频率两组间无显著差异 ;在ε4携带情况下 ,不同ACE基因型冠心病危险度不同 ,DD型 >ID型 ,而Ⅱ型似有一定的保护作用。结论 :ε4等位基因是冠心病的遗传易患因子 ;ACE基因与冠心病发病无显著关联 ,但对ε4等位基因有一定的“修饰”作用。
AIM: To investigate the association of ApoE polymorphism and angiotensin converting enzyme (ACE) gene insertion / deletion (I / D) polymorphisms with coronary heart disease in Chinese. Methods: ApoE and ACE genotypes were detected in PCR-RFLP and PCR in 129 patients with CHD and 90 healthy controls. Results: The frequencies of ε3 / 4 genotype and ε4 allele in CHD group were significantly higher than those in control group. The frequency of ε3 / 3 genotype and ε3 allele in CHD group were significantly lower than those in control group. The frequencies of ACE genotype and allele There was no significant difference between the two groups. In the case of ε4, the risk of coronary heart disease was different for different ACE genotypes, DD type> ID type, and Ⅱ type had some protective effect. CONCLUSION: The ε4 allele is a genetic predisposition to coronary heart disease. ACE gene is not associated with the incidence of coronary heart disease, but has some “modification” effect on the ε4 allele.