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目的探讨syndecan-1和乙酰肝素酶(heparanase)mRNA在胃癌组织中的表达及其与胃癌转移和预后的关系。方法采用原位杂交方法,检测118例胃癌组织中syndecan-1和乙酰肝素酶mRNA的表达水平。结果118例胃癌组织中syndecan-1 mRNA及乙酰肝素酶mRNA的阳性率分别为42．4％和55．9％;syndecan-1 mRNA及乙酰肝素酶mRNA阳性表达率与肿瘤浸润深度(X2= 32．95,P=0．001;X2=23．19,P=0．001)、脉管侵犯(X2=46．22,P=0．001;X2=33．78,P=0．001)、淋巴结转移(X2=28．62,P=0．001;X2=25．43,P=0．001)和远处转移(X2=63．30,P=0．001;X2= 65．76,P=0．001)密切相关;而syndecan-1 mRNA的表达还与肿瘤大小有相关性(X2=6．25,P= 0．012)。Syndecan-1 mRNA表达水平与乙酰肝素酶mRNA表达呈负相关(r=-0．844,P=0．001); Syndecan-1 mRNA低表达病例平均生存时间和5年生存率低于高表达组,乙酰肝素酶mRNA阳性表达病例的平均生存时间和5年生存率均明显低于阴性表达的病例。结论Syndecan-1 mRNA低表达和乙酰肝素酶mRNA高表达参与胃癌的浸润和转移过程,是指导临床治疗及估计预后的有意义指标。
Objective To investigate the expression of syndecan-1 and heparanase mRNA in gastric carcinoma and its relationship with metastasis and prognosis of gastric carcinoma. Methods The expression of syndecan-1 and heparanase mRNA in 118 cases of gastric cancer tissues were detected by in situ hybridization. Results The positive rates of syndecan-1 mRNA and heparanase mRNA in 118 cases of gastric cancer tissues were 42.4% and 55.9% respectively. The positive rates of syndecan-1 mRNA and heparanase mRNA were significantly correlated with the depth of tumor invasion = 32.95, P = 0.001; X2 = 23.19, P = 0.001), vascular invasion (X2 = 46.22, P = 0.001; 001), lymph node metastasis (X2 = 28.62, P = 0.001; X2 = 25.43, P = 0.001) and distant metastases (X2 = 63.30, P = 0.001; .76, P = 0.001). The expression of syndecan-1 mRNA was also correlated with tumor size (X2 = 6.25, P = 0.012). Syndecan-1 mRNA expression was negatively correlated with heparanase mRNA expression (r = -0.844, P = 0.001). The mean survival time and 5-year survival rate of patients with low expression of Syndecan-1 mRNA were lower than those of high expression Group, the average survival time and 5-year survival rate of heparanase mRNA positive expression were significantly lower than the negative expression of the cases. Conclusions The low expression of Syndecan-1 mRNA and the high expression of heparanase mRNA are involved in the invasion and metastasis of gastric cancer. It is a meaningful indicator to guide the clinical treatment and prognosis.