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为了解运动创伤中肌肉、肌腱、关节囊中异位化骨的机制及软骨细胞在此过程中所起的作用,本研究用牛骨形态发生蛋白(bBMP)诱导体外不传代培养的胎儿关节软骨细胞,发现:在bBMP的诱导下,不传代培养的软骨细胞逐渐变为成骨细胞样形态,它们表达的碱性磷酸酶明显增加,细胞周围的钙沉积和基质沉积也明显增加。在bBMP的诱导下,关节软骨细胞开始表达具有成骨细胞特异性和骨基质特异性的骨钙素。这些结果表明,在bBMP的诱导下,胎儿关节软骨细胞已开始表达成骨细胞表型,并向成骨细胞样细胞方向分化。本研究提示,在异位化骨和各种软骨内化骨过程中,软骨细胞可能具有较活跃的参与骨形成的作用,可能不像以前所假设的那样仅分化为肥大软骨细胞,然后变性,最终坏死。
In order to understand the mechanism of ectopic bone in muscle, tendon and joint capsule during traumatic injury and the role of chondrocytes in this process, bBMP was used to induce fetal articular cartilage The results showed that under the induction of bBMP, the non-subcultured chondrocytes gradually changed into osteoblast-like morphology. The expression of alkaline phosphatase was significantly increased, and the deposition of calcium and the deposition of matrix around the cells were also significantly increased. Under the induction of bBMP, articular chondrocytes begin to express osteocalcin with osteoblast specificity and bone matrix specificity. These results indicate that fetal articular chondrocytes have begun to express osteoblast phenotypes induced by bBMP and differentiate into osteoblast-like cells. The present study suggests that chondrocytes may play a more active role in bone formation during ectopic bone and various cartilage internalization and may not differentiate into hypertrophic chondrocytes just as previously assumed and then degenerate, Eventually necrosis.