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目的 探讨钙结合蛋白D28k(CaBP)在帕金森病(PD)发病机制中的作用。方法 将1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)按30mg/kg体重腹腔注射给C57BL小鼠,建立PD鼠模型。用反转录PCR及原位分子杂交方法检测PD鼠和正常鼠脑内CaBP mRMA的表达变化。结果 与正常鼠对比,CaBP mRNA在PD鼠的海马、纹状体、黑质等脑区的表达水平明显下调。结论 CaBP可能通过对特定脑区神经元的保护作用来抵御PD的发生。
Objective To investigate the role of calcium binding protein D28k (CaBP) in the pathogenesis of Parkinson’s disease (PD). Methods 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) was intraperitoneally injected into C57BL mice at a dose of 30mg / kg to establish a model of PD mice. Reverse transcriptional PCR and in situ hybridization were used to detect the expression of CaBP mRMA in the brains of PD and normal mice. Results Compared with normal mice, the expression of CaBP mRNA in hippocampus, striatum, substantia nigra and other brain regions was significantly down-regulated. Conclusion CaBP may resist the occurrence of PD through the protection of neurons in specific brain regions.