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本文旨在观察当细粒棘球蚴(HF)和猪囊尾蚴(CF)作为抗原时,棘球蚴病和猪囊尾蚴病患者之间血清交叉反应的频率,并且探索这种抗原共同和特异的成份。按Cho等的方法对5例细粒棘球蚴病患者的血清,67例脑囊虫病患者的血清,作免疫诊断。另外,以同法对29例肺吸虫病、25例裂头蚴病、25例支睾吸虫病和10例绦虫病患者的血清进行测定。用ELISA法测定细粒棘球蚴病和猪囊尾蚴病患者的特异抗体,先将蛋白浓度为2.5μg/ml的HF和CF分别置于有盖培养皿内,4℃过夜。以1:100稀释病人的血清,于36℃反应2h。过氧化物酶结合的IgG以1:10000稀释,含邻苯二胺的底物在20℃反应30分钟。在波长为492nm处,棘球蚴病
This article aims to investigate the frequency of serum cross-reactivity between patients with cysticercosis and cysticercosis when Echinococcus granulosus (HF) and Cysticercus cellulosae (CF) were used as antigens, and to explore the common and specific The ingredients. According to Cho and other methods of 5 cases of patients with echinococcosis serum, 67 cases of cysticercosis patients serum for immunological diagnosis. In addition, the same law on 29 cases of paragonimiasis, 25 cases of sparganosis, 25 cases of clonorchiasis and 10 cases of taeniasis in patients with serum were measured. Specific antibodies against patients with granulomatous echinococcosis and cysticercosis were assayed by ELISA. HF and CF with a protein concentration of 2.5 μg / ml were placed in covered petri dishes at 4 ° C overnight. The patient’s serum was diluted 1: 100 and reacted for 2h at 36 ° C. Peroxidase-conjugated IgG was diluted 1: 10000 and substrates containing o-phenylenediamine were reacted at 20 ° C for 30 minutes. At 492 nm wavelength, hydatid disease