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利用羟丙基纤维素溶液动态涂层技术修饰毛细管管壁 ,改善了分离效率 .在不影响质谱检测的条件下 ,将动态涂层毛细管电泳与质谱检测联用 ,有效地提高了对蛋白质的鉴定能力 .将该技术应用于对复杂蛋白质样品的酶解产物的分析鉴定 ,结果令人满意
Dynamic coating of hydroxypropyl cellulose solution was used to modify the capillary wall to improve the separation efficiency.Under the condition of not affecting the mass spectrum detection, the combination of dynamic coating capillary electrophoresis and mass spectrometry detection effectively improved the identification of protein Ability to apply this technique to the analysis and identification of enzymatic products of complex protein samples with satisfactory results