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为了简化人类蜕膜细胞体外培养技术 ,采用复合消化酶改进蜕膜组织的消化过程 ,全组份蜕膜细胞培养加传代简化蜕膜基质细胞 (decidual stromal cell,DSC)提纯、泌乳素 (prolactin,PRL)免疫组化检测培养细胞组成。所采用的消化方法具有耗时少、消化彻底、活细胞率高等优点。PRL 免疫组化显示 95 %蜕膜细胞为 DSC。结果提示 :实验方法简单、经济、实用 ,简化了原来复杂的 DSC提纯程序。
In order to simplify the culture of human decidual cells in vitro, the compound digestive enzymes were used to improve the digestive process of decidual tissue. All the decidual cells were cultured and subcultured to simplify the decidual stromal cells (DSC). Prolactin PRL) immunohistochemistry of cultured cells. The digestion method used has the advantages of less time consuming, thorough digestion and higher living cell rate. PRL immunohistochemistry showed that 95% of the decidual cells were DSC. The results suggest that the experimental method is simple, economical and practical, simplifying the original complex DSC purification program.