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目的:研究舌癌脑转移细胞系Tb的转移相关生物学特性。方法:分别用软琼脂糖克隆形成法、流式细胞术、癌细胞悬液经探鼠尾静脉注射法(每只鼠0.2ml,含细胞2×105)检测舌癌细胞系Tea8113(T)和Tb细胞的克隆形成率、P53蛋白和nm23-H1蛋白表达率、Tb细胞在肺内形成转移灶的组织学过程以及T和Tb细胞在脑表面的人工转移力。结果:T和Tb细胞的克隆形成率分别为19.5%和35.5%;P53蛋白表达率分别为37.6%和24.9%,nm23-H1蛋白表达率分别为100%和99.9%;Tb细胞在肺内先形成小的痕控,逐渐增大,最终形成巨大转移灶并产生胸水,转移灶仍保持鳞状上皮癌组织学特征;T和Tb细胞在裸鼠肺表面形成的转移结数分别为25±16和73±41。结论:Tb细胞转移力强于T细胞。
Objective: To study the metastasis-related biological characteristics of tongue metastasis cell line Tb. Methods: The tongue cancer cell line Tea8113 (T) was detected by soft agarose colony formation, flow cytometry and cancer cell suspension by tail vein injection (0.2ml per mouse, containing 2 × 105 cells) And Tb cells, the expression of P53 protein and nm23-H1 protein, the histological process of Tb cells forming metastasis in the lung, and the artificial transfer of T and Tb cells on the brain surface. Results: The clonogenic rates of T and Tb cells were 19.5% and 35.5% respectively; the expression rates of P53 protein were 37.6% and 24.9%, respectively; the expression rates of nm23-H1 protein were 100% and 99.9% respectively; Tb cells in the lung first to form a small trace control, and gradually increased, eventually forming a huge metastasis and the formation of pleural effusion, metastasis still maintain the histological features of squamous cell carcinoma; T and Tb cells in the nude mouse lung metastasis junction The numbers were 25 ± 16 and 73 ± 41, respectively. Conclusion: Tb cells are stronger than T cells.