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因缺乏特异性血清学检测方法,过去对戊型肝炎的诊断多依靠排除法,即排除急性甲型和乙型肝炎的方法。所以对戊型肝炎在急性散发性肝炎发病中的地位缺乏深入了解。作者应用一种新的蛋白印迹法(WesternBlot法)检测戊型肝炎病毒(HEV)抗体(抗-HEV)并对苏丹卡图木地区39例急性小儿肝炎患者进行了病因学分析。该方法是先将编码HEV一种主要结构蛋白的基因片段插入到质粒pATH_(10)色氨酸合成酶基因E(TrpE)的读码框架中,然后将此质粒引入埃希氏大肠杆菌DH_6αF′株,使TrpE-HEV融合蛋
Due to the lack of specific serological tests, the diagnosis of hepatitis E in the past rely mostly on the exclusion method, that is, the method of excluding acute hepatitis A and B. Therefore, the lack of a thorough understanding of the status of hepatitis E in the pathogenesis of acute sporadic hepatitis. The authors used a new Western blot (Western Blot method) to detect hepatitis E virus (HEV) antibody (anti-HEV) and the etiology of 39 cases of acute pediatric hepatitis in Catukawa region of Sudan. In this method, a gene fragment encoding a major structural protein of HEV is inserted into the reading frame of the plasmid pATH_ (10) tryptophan synthase gene E (TrpE), and then the plasmid is introduced into Escherichia coli DH_6αF ’ Strain, TrpE-HEV fusion protein