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从人外周血淋巴细胞中克隆了氨肽酶N基因(APN)的上游启动子并进行了序列分析. 构建了含APN上游启动子和荧光酶报告基因的重组质粒pXP1-APNLuc, 对骨髓母细胞和几种肿瘤细胞进行转染. 分析结果表明APN上游启动子具有骨髓特异性, 其在骨髓母细胞KG1a中活性最高, 而在Jurkat细胞中活性很低. APN启动子在肺腺癌细胞中活性较高, 在肝癌细胞中基本无活性, 在舌癌细胞和食管癌细胞中活性明显降低. APN基因启动子的这一特点为在肿瘤患者实施化、放疗的同时, 特异性地保护造血系统提供了新的思路.
The upstream promoter of the aminopeptidase N gene (APN) was cloned from human peripheral blood lymphocytes and subjected to sequence analysis. A recombinant plasmid pXP1-APNLuc containing an APN upstream promoter and a luciferase reporter gene was constructed, and the myeloid mother cell was constructed. Transfection with several tumor cells. The analysis results showed that the APN upstream promoter has bone marrow specificity, its activity is highest in the bone marrow blast cells KG1a, and its activity in Jurkat cells is very low. APN promoter activity in lung adenocarcinoma cells Higher, basically inactive in hepatocellular carcinoma cells, significantly reduced activity in tongue cancer cells and esophageal cancer cells. This feature of the APN gene promoter specifically protects the hematopoietic system while performing chemotherapy and radiotherapy in patients with tumors. New ideas.