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采用滤膜法对2012年北极黄河站科学考察采集的新奥尔松地区8个鹿粪和1个鸟粪样品进行分离鉴定,共获得84株肠球菌和85株大肠杆菌。84株肠球菌分属于10个属,其中屎肠球菌(28株)、鼠肠球菌(28株)及鸟肠球菌(10株)为优势菌。采用K-B纸片法对受试菌株进行药敏试验结果显示:85株大肠杆菌对利福平全部耐药,对氨苄西林的耐药率为81%;对甲氧苄啶(TMP)、喹诺酮类的萘啶酸(NAL)与环丙沙星(CIP)、四环素(TCY)4种抗生素高度敏感。84株肠球菌中屎肠球菌对氨苄西林耐药率最高(89%),对万古霉素耐药率最低(4%)。10株鸟肠球菌对利福平全部耐药,而28株鼠肠球菌对利福平耐药率最低(4%),对链霉素耐药率最高(75%)。18株其他肠球菌对利福平耐药率最高(61%)。上述结果表明抗生素耐药性已出现于北极新奥尔松地区野生动物肠道菌群中。
Eight deer samples and one sample of bird droppings from New Arsson collected by scientific investigation of the Arctic Yellow River Station in 2012 were isolated and identified. 84 enterococci and 85 Escherichia coli strains were obtained. Enterococcus faecium belonged to 10 genera, of which Enterococcus faecium (28 strains), Enterococcus spp. (28 strains) and Enterococcus faecium (10 strains) were dominant bacteria. The susceptibility test of the tested strains by KB paper method showed that 85 strains of Escherichia coli were completely resistant to rifampicin, and the resistance rate to ampicillin was 81%. The inhibition rates of trimethoprim (TMP), quinolones Nalidixic acid (NAL) and ciprofloxacin (CIP), tetracycline (TCY) four kinds of antibiotics highly sensitive. Enterococcus faecium was the most resistant to ampicillin in 84 Enterococcus isolates (89%), and vancomycin was the lowest (4%). Ten strains of enterococci were completely resistant to rifampicin, while the 28 strains of enterococci showed the lowest rates of resistance to rifampin (4%) and the highest rates of streptomycin (75%). 18 strains of other enterococci were the most resistant to rifampin (61%). The above results indicate that antibiotic resistance has emerged in the intestinal flora of wildlife in the New Alesund region of the Arctic.