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药物代谢是肝细胞的重要生物功能之一.通过测定肝细胞培养液中某一物质的浓度变化,可准确反映肝细胞代谢功能,而国内尚无这方面报道.为此我们参照国外人工肝研究经验,选择安定为指标,通过研究,找到一种培养人肝细胞药物代谢功能的测定方法.1 材料和方法1.1 材料 L-15培养基为 Gibco 公司产品.752型紫外分光光度仪系上海第三分析仪器厂产品.精密称取安定20 mg(干燥
Drug metabolism is one of the important biological functions of hepatocytes.Through measuring the change of the concentration of a substance in the hepatocyte culture medium, the metabolic function of hepatocytes can be accurately reflected, but there is no report about this in our country.Therefore, we refer to the foreign study of artificial liver Experience, choose stability as an indicator, through research to find a method of culture of human liver cell metabolism function.1 Materials and Methods 1.1 Materials L-15 medium for Gibco products .752 UV spectrophotometer in Shanghai third Analysis of plant products. Precision weighed and stability 20 mg (dry