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以秋水仙素三种浓度处理两个组合的春小麦杂种F_1代种子,再分别用~(60)Coγ射线5种剂量辐照,结果γ射线对观察性状的诱变效应均极显著。秋水仙素仅对一个组合的株高和主穗长有诱变作用,表明秋水仙素的诱变作用受基因型和春小麦生育期的影响。而秋水仙素与~(60)Coγ射线的交互作用和秋水仙素的作用一致,说明只有在秋水仙素诱变作用有效时,它与γ射线的互作才可能显著。同质性方差分析表明,两个组合5个性状(株高、主穗长、小穗数、每穗粒数和小穗粒数)的变异无差异,这表明复合处理不受基因型和材料的影响。若以秋水仙素和~(60)Coγ射线两因素处理的变异系数为指标,则新春2号×761的适宜处理法为α_2(秋水仙素400mg/kg)×b_1(~(60)Coγ射线10krad),而Pavan×761的适宜处理方法为α_2(秋水仙素400mg/kg)×b_1(~(60)Coγ射线5krad)。
Two combinations of spring wheat F 1 seeds were treated with colchicine at different concentrations, and then irradiated with ~ (60) Coγ-rays at different doses respectively. The results showed that the mutagenic effects of γ-rays on the observed traits were all significant. Colchicine only mutated the plant height and the main spike length of one combination, indicating that the mutagenesis of colchicine is influenced by genotype and spring wheat growth period. However, the interaction between colchicine and ~ (60) Coγ-ray was consistent with that of colchicine, indicating that its interaction with γ-ray could be significant only when the colchicine mutation was effective. Homogeneity analysis of variance showed no significant difference in the variability among the five traits (plant height, spike length, spikelet number, grains per spike and spikelet number) in the two combinations, indicating that the composite treatment was not affected by genotype and material Impact. When the coefficient of variation of colchicine and ~ (60) Coγ-ray treatment were used as indexes, the suitable treatment method of Xinchun 2 × 761 was α_2 (colchicine 400mg / kg) × b_1 (~ (60) Coγ ray 10krad), while Pavan × 761 is suitable for α_2 (colchicine 400mg / kg) × b_1 (~ (60) Coγ ray 5krad).