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应用制备电泳技术，从大阪鲫（ＣａｒａｓｓｉｕｓａｕｒａｔｕｓＣｕｖｉｅｒ）卵匀浆液中提纯两种卵黄蛋白—蛋白质Ｈ及蛋白质Ｌ。蛋白质Ｈ类似卵黄脂磷蛋白（磷为０．２４％，氮为９．１８％，等电点６．７４），分子量４７１０００。在ＳＤＳ电泳中显示４条带，分子量分别为９５８００、９０７００、８６５００、２６９００。蛋白质Ｌ是类卵黄高磷蛋白，其分子量为９４０００。在ＳＤＳ电泳中显示一条带（含磷８．８５％、氰１４．１４％，等电点７．０１），分子量２９４００。同时也观察到在不同ｐＨ值溶液中两种蛋白质紫外光吸收光谱和四阶导数谱变化的现象。
Two kinds of yolk proteins-protein H and protein L were purified from the homogenate of Carassius auratus Cuvier by preparative electrophoresis. Protein H is similar to yolk phophorophore (phosphorus 0.24%, nitrogen 9.18%, isoelectric point 6.74) and molecular weight 471000. In the SDS electrophoresis showed four bands, molecular weight was 95800,90700,86500,29,600. Protein L is a phosvitin-like protein with a molecular weight of 94,000. A band (8.85% phosphorus, 14.14% cyanide, isoelectric point 7.01) showed a molecular weight of 29,400 in SDS electrophoresis. At the same time, we also observed the changes of ultraviolet absorption spectra and fourth derivative spectra of two proteins in different pH solutions.