论文部分内容阅读
目的:采用细胞培养的方法,体外观察黄芩苷、芍药苷及其联合用药对转化生长因子β1(TGFβ1)诱导的肝星状细胞(HSC-T6)增殖作用的影响。方法:采用TGFβ1诱导HSC-T6细胞作为活化的肝星状细胞的研究模型,在倒置显微镜下观察细胞形态变化并采用噻唑蓝(MTT)法测定黄芩苷、芍药苷及其联合用药对TGFβ1诱导的HSC-T6细胞增殖的影响。结果:黄芩苷高(0.981±0.081)、低剂量组(1.046±0.073)和芍药苷高(1.019±0.111)、低剂量组(0.983±0.064)对TGFβ1诱导的HSC-T6细胞活化有一定的抑制作用,差异无统计学意义(P>0.05)。联合用药中,高剂量黄芩苷+低剂量芍药苷(0.921±0.076)和高剂量黄芩苷+高剂量芍药苷(0.893±0.087)与模型组(1.108±0.098)相比,OD值明显降低(P<0.05)。倒置显微镜显示,各组药物除了引起部分细胞死亡外,对细胞形态无明显影响。结论:黄芩苷、芍药苷对TGFβ1的促诱导HSC-T6细胞活化作用增殖有一定的抑制作用,并且黄芩苷与芍药苷联合用药可明显提高对TGFβ1诱导HSC-T6细胞增殖抑制作用。
OBJECTIVE: To observe the effects of baicalin, paeoniflorin and their combination on the proliferation of hepatic stellate cells (HSC-T6) induced by transforming growth factor β1 (TGFβ1) in vitro. Methods: TGFβ1 was used to induce HSC-T6 cells as activated hepatic stellate cells. Morphological changes were observed under an inverted microscope. The effects of baicalin, paeoniflorin and their combination on TGFβ1-induced HSC-T6 cells proliferation. Results: The inhibition of TGFβ1-induced activation of HSC-T6 cells by baicalin (0.981 ± 0.081), low dose (1.046 ± 0.073) and paeoniflorin (1.019 ± 0.111) and low dose (0.983 ± 0.064) Role, the difference was not statistically significant (P> 0.05). Compared with model group (1.108 ± 0.098), the OD value of high dose baicalin + low dose paeoniflorin (0.921 ± 0.076) and high dose baicalin + high dose paeoniflorin (0.893 ± 0.087) <0.05). Inverted microscopy showed that in addition to causing some cell death in each group of drugs, no significant effect on cell morphology. CONCLUSION: Baicalin and paeoniflorin can inhibit the proliferation of HSC-T6 cells induced by TGFβ1, and the combination of baicalin and paeoniflorin can significantly inhibit the proliferation of HSC-T6 induced by TGFβ1.