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目的:探讨高效液相色谱-质谱法在中成药分析中的应用效果。方法:建立六味地黄丸中4种磺胺类药物残留的高效液相色谱-质谱方法,采用QuattroUltimamPt质谱仪进行分离,甲醇-水作为流动相,2.5mmol/L甲酸胺-0.1%的甲酸作为流动相添加剂,梯度洗脱。结果:检测方法回收率在65.2%~112.2%之间,方法检出限为0.01~1.0ng/g,方法精密度为1.0%~17.6%。结论:高效液相色谱-质谱法在中成药中相关残留的检测具有灵敏度高、回收率高、重现性好等优势,能促进我国中药质量的提高。
Objective: To investigate the application effect of high performance liquid chromatography-mass spectrometry in the analysis of proprietary Chinese medicines. Methods: To establish a HPLC-MS method for the determination of four sulfonamides residues in Liuweidihuangwan. The separation was performed on a QuattroUltimamPt mass spectrometer using methanol-water as mobile phase and 2.5mmol / L formic acid-0.1% formic acid as mobile phase Additives, gradient elution. Results: The recoveries were between 65.2% and 112.2%. The limits of detection were 0.01 ~ 1.0ng / g. The method precision was 1.0% ~ 17.6%. Conclusion: HPLC-MS method has the advantages of high sensitivity, high recovery, good reproducibility and so on, which can promote the improvement of quality of Chinese traditional medicine.