【摘 要】
：
A protein named NVP(1) was isolated from Nidus vespae.The aim of the present study was to elucidate whether and how NVP(1) modulates the proliferation of HepG2
【机 构】
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China-UK HUST-RRes Genetic Engineering and Genomies Joint Laboratory, the Key Laboratory of Molecula
论文部分内容阅读
A protein named NVP(1) was isolated from Nidus vespae.The aim of the present study was to elucidate whether and how NVP(1) modulates the proliferation of HepG2 cells. NVP(1)at a concentration of 6.6 μg/ml could arrest the cell cycle at stage G1 and inhibit the mRNA expression ofcyclinB, cyclinD1 and cyclinE. NVP(1) suppressed cdk2 protein expression,but increased p27 and p21 protein expression. However,NVP(1) did not alter p16 protein expression levels. NVP(1)promoted apoptosis in HepG2 cells as indicated by nuclear ehromatin condensation, and in addition, the extracellular signal-regulated kinase (ERK) signaling pathway was activated. Moreover, the p-ERK protein expression level was attenuated when the HepG2 cells were pretreated with ERK inhibitor PD98059. These results demonstrate that NVP(1)inhibits proliferation of HepG2 through ERK signaling pathway. NVP(1) could be a potential drug for liver cancer.
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