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本文从室内培养的7日龄玉米幼苗中提取、分离、鉴定了抗性次生化合物丁布(2,4-d ihydroxy-7-m ethoxy-2H-1,4-benzoxazin-3(4H)-one,D IMBOA),并就该物质对玉米纹枯病病原菌立枯丝核菌(Rhizoctonia solani)的活性与三种酚酸类物质(阿魏酸、对羟基肉桂酸和咖啡酸)进行了离体比较研究。结果表明,丁布(D IMBOA),对立枯丝核菌有很强的生物活性,在浓度为50μg/mL时即可抑制立枯丝核菌菌丝的生长,抑制率为18.52%。阿魏酸、对羟基肉桂酸和咖啡酸,这三种酚酸在浓度250μg/mL时对立枯丝核菌菌丝的生长有抑制作用,抑制率分别为26.30%、8.50%和6.30%。不仅如此,丁布与对羟基肉桂酸之间、以及三种酚酸两两组合之间还存在一定的协同作用。在浓度相等的情况下,丁布与对羟基肉桂酸的等量混合液的抑菌率显著高于这两种物质单独存在时的抑菌率之和;同样,对羟基肉桂酸与阿魏酸的等量混合液的抑菌率比单一的对羟基肉桂酸溶液的抑菌率高18.89%,比单一的阿魏酸溶液的抑菌率高13.33%;对羟基肉桂酸与咖啡酸的等量混合液,抑菌率比两者单独试验时分别高9.63%和14.83%;阿魏酸与咖啡酸的混合液,抑菌率比两酸单独试验时分别高11.48%和22.23%。这一结果提示植物体内产生适当比例不同次生化合物的组合对植物抗病性的提高是至关重要的。
In this study, we isolated, isolated and identified the 7-day-old corn seedlings cultivated indoors and studied the effects of 2,4-dhydroxy-7-methoxy-2H-1,4-benzoxazin- one, D IMBOA) and the activity of the substance against Rhizoctonia solani, a pathogen of the sheath blight of corn, with three phenolic acids (ferulic acid, p-hydroxycinnamic acid and caffeic acid) Comparative study of body. The results showed that DIBMBOA had a strong biological activity against Rhizoctonia solani and inhibited the growth of Rhizoctonia solani at a concentration of 50μg / mL with the inhibition rate of 18.52%. Ferulic acid, p-hydroxycinnamic acid and caffeic acid. These three phenolic acids inhibited the growth of Rhizoctonia solani at the concentration of 250μg / mL with the inhibitory rates of 26.30%, 8.50% and 6.30%, respectively. Not only that, there is still some synergy between butyric and p-hydroxycinnamic acid, and the combination of three phenolic acids. In the case of equal concentration, the antibacterial rate of the mixture of butyric and p-hydroxycinnamic acid was significantly higher than the sum of the antibacterial activity of the two substances when they existed alone. Similarly, The antibacterial activity of the mixture of the same amount is higher than that of the single p-hydroxycinnamic acid solution by 18.89%, which is 13.33% higher than that of the single ferulic acid solution. The equivalent amount of hydroxycinnamic acid and caffeic acid The antibacterial activity of the mixture was 9.63% and 14.83% higher than that of the mixture alone. The mixture of ferulic acid and caffeic acid had 11.98% and 22.23% higher antibacterial activity than the two acids alone. This result suggests that a combination of different secondary compounds produced in appropriate proportions in plants is crucial for the improvement of plant disease resistance.