真菌的产孢状,包括孢子在孢子梗上的着生及其排列,是部分属(尤其是半知菌类)鉴定和分类的主要依据。观察完整的真菌产孢状,是真菌研究和某些植物病害鉴定中的一个重要组成部分,从培养基或植物病部直接挑菌镜检,常会破坏孢子着生状态的完整性,特别是分生孢子形成后极易脱落的种类,必须采用特别的培养方式,使孢子从形成到镜检不受到大的触动,才能观察到完整的真菌产孢状。常规方法是进行真菌的玻片培养(见方中达,1977,植病研究方法),但这种方法需对载玻片等进行严格消毒,还要用无菌吸水纸及无菌水等来保湿培养。为此,作者试将原玻片培养法改进成为一种更简便、实用的真菌产孢状观察法。经过对从木瓜病果分离的6个半知菌属的代表种的观察,效果甚佳。它能完整地观察到真菌的产孢状,真菌菌丝融合现象和菌核形成初期的菌丝体变化等。现将方法介绍如下: Fungal sporulation, including spores in the spore stalk on the students and their arrangement, is part of the genus (especially semi-known fungi) identification and classification of the main basis. Observation of intact fungal sporulation is an important part of fungal research and identification of certain plant diseases. Direct microscopic examination of the bacteria from the culture medium or plant diseases often ruins the integrity of the sporulation state, especially After the formation of spores can easily fall off the species, we must adopt a special culture, so that spores from the formation to the microscopic examination did not receive a big touch in order to observe the full fungal sporulation. Conventional methods are fungal slides (see Fang Zhongda, 1977, Phytosanitary Methods), but this method requires strict disinfection of slides and the like, as well as moisturizing with sterile absorbent paper and sterile water to cultivate. To this end, the author tried to improve the original slide culture method to become a more simple and practical fungus spore-like observation. Observed from the representative of six semi-Amycolata isolated from papaya disease fruit, the effect is very good. It can be completely observed fungal sporulation, fungal mycelium fusion phenomenon and the initial formation of sclerotia mycelium changes. The method is introduced as follows: