目的 :探讨端粒酶活性和膀胱癌之间的关系。方法 :采用在PCR基础上建立的TRAP ELISA法对30例膀胱癌及 9例切缘组织和 7例正常膀胱组织中端粒酶活性进行半定量的研究。结果 :膀胱癌组总阳性率为83.3% (2 5 / 30 ) ,与切缘组 11.1% (1/ 9)和对照组 0 .0 % (0 / 7)相比差异有极显著性意义 (P <0 .0 1) ;后两者之间差异无显著性意义 (P >0 .0 5 )。端粒酶阳性表达率和表达强度与患者年龄、性别、病变部位、肿瘤大小、手术方式等无显著性相关 (P >0 .0 5 ) ,而端粒酶表达强度与细胞病理分级具有相关性 (P <0 .0 5 )。结论 :膀胱肿瘤的端粒长度和端粒酶活性对于判断疾病的恶性程度、预后、监测微小残瘤病灶和预示早期复发均有积极的意义 Objective: To investigate the relationship between telomerase activity and bladder cancer. Methods: The telomerase activity in 30 cases of bladder cancer, 9 cases of marginal tissue and 7 cases of normal bladder tissue were studied by TRAP ELISA based on PCR. Results: The positive rate of bladder cancer was 83.3% (25/30), which was significantly different from that of the control group (11.1%, 1/9) and the control group (0. 0%) (0/7) P <0.01). There was no significant difference between the latter two groups (P> 0.05). There was no significant correlation between the positive expression rate of telomerase and the expression intensity in patients with age, sex, lesion location, tumor size, operation method and so on (P> 0.05), but the correlation between telomerase expression and cell pathological grade (P <0. 05). Conclusion: The telomere length and telomerase activity of bladder tumor have a positive meaning for judging the malignancy of the disease, prognosis, monitoring the lesion of the small tumor and predicting the early recurrence