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以一端连有单电子转移自由基聚合(RAFT)链转移剂的聚乙二醇(PEG)为大分子链转移剂,调控2-(4-羟基丁酰氧基)甲基丙烯酸叔丁酯(t BHBMA)的RAFT聚合,得到的PEG-b-Pt BHBMA嵌段共聚物引发丙交酯的开环聚合,制得接枝共聚物PEG-b-(Pt BA-g-PLA).通过聚乳酸末端的羟基与7-甲氧基香豆素-3-羧酸(COU)中羧基的酯化反应,得到了含有荧光标记分子的接枝共聚物PEG-b-(Pt BA-g-PLA-COU).该聚合物主链选择性水解,得到了含有荧光标记分子的两亲性接枝共聚物PEG-b-(PAA-g-PLA-COU).以PEG-b-(PAA-g-PLA-COU)为药物载体,对阿霉素(DOX)进行了负载,制得了含有荧光标记分子的聚合物载药胶束.利用紫外光谱和动态光散射测定了载药胶束的载药量和胶束尺寸.
Polyethylene glycol (PEG) linked to one electron transfer radical polymerization (RAFT) chain transfer agent as a macromolecular chain transfer agent regulates tert-butyl 2- (4-hydroxybutyryloxy) t BHBMA), and the resulting PEG-b-Pt BHBMA block copolymer initiated the ring-opening polymerization of lactide to prepare the graft copolymer PEG-b- (Pt BA-g-PLA) The terminal hydroxyl group was esterified with the carboxyl group in 7-methoxycoumarin-3-carboxylic acid (COU) to obtain PEG-b- (Pt BA-g-PLA- (PAA-g-PLA-COU) .After the hydrolysis of the polymer backbone, the amphiphilic graft copolymer PEG-b- PLA-COU) as drug carrier, loaded doxorubicin (DOX) to prepare polymer-loaded micelles containing fluorescent labeled molecules.The drug-loaded micelles were measured by ultraviolet spectroscopy and dynamic light scattering And micellar size.