论文部分内容阅读
为了提高大肠癌的早期诊断率,我们对结肠癌、结肠息肉、溃疡性结肠炎及结肠炎患者结肠镜检查并进行病理诊断,然后对 p53,DCC,APC,C-myc,K-ras 及 p73基因进行检测,并设正常对照组,探讨多个基因与大肠癌的关系和早期诊断意义.1 材料和方法1.1 材料 1999-03/1999-10我院进行肠镜检查取结肠癌组织40例,结肠息肉组织49例,溃疡性结肠炎组织12例,结肠炎6例及正常对照组13例.年龄5岁～81岁,平均49岁.男87例,女33例.酚、氯仿、无水乙醇、蛋白酶 K、异丙醇等化学试剂购自原平试剂公司;mRNA 提取采用 Promeg 公司 PolyATtract
To improve the early diagnosis rate of colorectal cancer, we performed colonoscopy and pathological diagnosis of patients with colon cancer, colon polyps, ulcerative colitis, and colitis, and then performed p53, DCC, APC, C-myc, K-ras, and p73. The gene was tested and a normal control group was set up to explore the relationship between multiple genes and colorectal cancer and the significance of early diagnosis.1 Materials and methods 1.1 Materials 1999-03/1999-10 In our hospital, colonoscopy was used to collect 40 cases of colon cancer tissues. There were 49 cases of colon polyp tissue, 12 cases of ulcerative colitis, 6 cases of colitis, and 13 cases of normal control group. The age range was 5 years to 81 years old with an average age of 49 years. 87 males and 33 females. Phenol, chloroform, anhydrous Ethanol, proteinase K, isopropanol and other chemical reagents were purchased from Yuanping Reagent Co.; mRNA extraction was performed using Promeg PolyATtract.