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1877年Ehrlich首先记载了硷性苯胺染料有异染性特征。其后有许多学者对肥大细胞的异染性作了大量的研究。已知甲苯胺蓝、天青、美蓝、硫堇、结晶紫等多种硷性染料以及中性红等都能显示肥大细胞。我们从1953年起,20多年来一直使用吉姆沙氏液染小白鼠的肥大细胞,方法简便,效果良好,满足了教学需要,兹介绍如下。方法 1.将小白鼠击头杀死或一手按头一手拉尾处死后,用水打湿腹壁皮肤后再剪开,取出小块皮下疏松结缔组织,放在干净的载玻片上,用分离针轻轻分离成铺片。
In 1877 Ehrlich first recorded the Alkaline Aniline dye is heterotropic. Since then, many scholars have done a lot of research on the metaplasia of mast cells. It is known that toluidine blue, azure, methylene blue, thionine, crystal violet and other alkaline dyes and neutral red can show mast cells. Since 1953, we have been using Jamsar stain to dye mast cells in mice for more than 20 years. The method is simple and effective and meets the teaching needs. Here we introduce the following. Method 1. Kill the mouse head or tail in the palm of your hand, wet the abdominal wall with water and then cut open, remove the loose subcutaneous loose connective tissue, on a clean glass slide with a light needle Light separated into pieces.