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目的分析肾综合征出血热(HFRS)患者和健康人群HLA-B及HLA-DQ基因区域的单核苷酸多态性(SNP)位点,探讨人感染汉坦病毒与其遗传特性间的关联性。方法采用等位基因特异性引物扩增(ASP-PCR)技术对HLA-B及HLA-DQ区域上的5个SNP位点进行扩增,利用病例对照研究探讨SNP与人群汉坦病毒感染状态之间的关系。结果筛选HFRS患者阳性标本24份,同时采集与病例相匹配的健康人群标本51份。rs34933313位点患者组和对照组的CC基因型频率分别为4.17%和29.41%,GG基因型为12.50%和15.69%,CG基因型为83.33%和54.90%,CC、GG基因型频率患者组低于对照组,CG基因型频率患者组高于对照组,且二者之间差异有统计学意义(χ2=7.050,P=0.029)。其他4个位点两组间的差异均无统计学意义。结论 rs34933313位点的基因多态性可能与人群汉坦病毒感染有关,可能会增加人群感染该病毒的风险。HLA-B基因可能与人群感染汉坦病毒存在相关性。
Objective To analyze single nucleotide polymorphism (SNP) loci in HLA-B and HLA-DQ gene regions of patients with hemorrhagic fever with renal syndrome (HFRS) and healthy people, and to investigate the association between Hantavirus and its genetic characteristics . Methods The SNPs of HLA-B and HLA-DQ were amplified by allele-specific primer-based amplification (ASP-PCR). A case-control study was conducted to investigate the association between SNP and Hantaviruses Relationship between. Results A total of 24 HFRS positive samples were screened, and 51 samples of healthy subjects were collected. The frequency of CC genotype in patients with rs34933313 locus was 4.17% and in controls was 29.41%, GG genotypes were 12.50% and 15.69%, CG genotypes were 83.33% and 54.90% In the control group, CG genotype frequency was higher in the patients than in the control group, and the difference was statistically significant (χ2 = 7.050, P = 0.029). The other four sites showed no significant difference between the two groups. Conclusion The genetic polymorphism of rs34933313 may be related to Hantavirus infection in human population, which may increase the risk of human infection with the virus. HLA-B gene may be associated with the human population infected with Hantavirus.