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对新鲜人胎盘进行硫酸铁分级沉淀，透析，DEAE-纤维素-23离子交换层析，制取初步纯化的核糖核酸酶抑制因子（RI）．小鼠急性毒性实验结果表明，RI对小鼠没有急性毒性．
Fresh human placenta was subjected to ferric sulfate fractionation, dialysis, and DEAE-cellulose-23 ion exchange chromatography to prepare a preliminary purified ribonuclease inhibitor (RI). The acute toxicity test in mice showed that RI had no acute toxicity to mice.