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用聚丙烯酰胺凝胶电泳法研究了干旱时棉花叶绿体易溶蛋白质和难溶蛋白质含量的变化。研究表明,试验植株叶绿体的蛋白质含量降低,且改变蛋白质的电泳谱,其变化依棉花水分亏缺时细胞中水的结构和状态而转移。干旱消除后,叶绿体易溶蛋白质含量甚至恢复到正常状态。虽然,这与其变性作用的可逆性有关。难溶蛋白质未恢复,可能是膜蛋白质的生物合成对干旱较为敏感。同时指出,蛋白质含量降低和蛋白质低分子部分的消失。应当指出,干旱时,棉花叶绿体蛋白质相应的氨基酸发生变化,而其组成成分未发生变化。
Polyacrylamide gel electrophoresis was used to study the changes of soluble protein and insoluble protein in chloroplasts of cotton during drought. Studies have shown that the chloroplast protein content of the test plants decreased, and changes in the electrophoretic pattern of the protein, the changes according to the cotton water deficit cells in the water structure and status of the transfer. After drought elimination, chloroplast soluble protein content even returned to normal. Although, this is related to the reversibility of its denaturation. Proteins that are sparingly soluble do not recover, probably due to drought-sensitive biosynthesis of membrane proteins. Also pointed out that the protein content decreased and the disappearance of low molecular weight protein. It should be pointed out that in drought, the corresponding amino acid changes of cotton chloroplast protein, but its composition has not changed.