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目的研究通过大鼠建立气管异位移植慢性闭塞性细支气管炎模型并测定不同阶段的白细胞介素17(IL-17)表达,分析其作用。方法 2015年6月至2016年6月采用大鼠建模的方法研究,将清洁级大鼠40对,随机分为两组,每组20对。其中A组采用Wistar大鼠制备供体,采用SD大鼠制备受体;B组均采用SD大鼠制备供体和受体,进行气管移植建模,分别在各时间段采样完成病理组织分析比较,以评估IL-17的表达和作用。结果实验组在第7、14、18天后病理切片上皮发生不同程度丢失,对照组未见明显改变;软骨破坏程度比较:第7天组间比较无显著差异,第14、28天实验组明显高于对照组(P<0.05);淋巴细胞计数比较:实验组在移植后第7、14、28天明显高于对照组(P<0.05);IL-17水平比较:两组在第7、14天组间无显著差异,第28天实验组IL-17水平较对照组明显升高,差异有显著性(P<0.05);且第28天实验组闭塞性细支气管炎发生率(100.0%)高于对照组的(0.00%)。结论通过建立大鼠异位气管移植慢性排斥反应模型,分析监测病理改变,证实异位气管移植后,IL-17的表达与闭塞性细支气管纤维化发展呈现正性相关。
OBJECTIVE: To establish a rat model of chronic bronchitis obliterans and determine the expression of interleukin-17 (IL-17) at different stages and analyze its role. Methods From June, 2015 to June, 2016, 40 rats in clean grade were randomly divided into two groups with 20 pairs in each group. In group A, Wistar rats were used to prepare donors and SD rats were used to prepare recipients. In group B, SD rats were used to prepare donors and recipients. Tracheal transplantation models were established. Pathological tissues were analyzed and compared at each time point To assess IL-17 expression and function. Results In the experimental group, the pathological sections of the experimental group were lost to varying degrees on the 7th, 14th and 18th days after the operation. There was no significant change in the control group. The degree of cartilage destruction was no significant difference between the 7th day and the 14th and 28th day (P <0.05). Compared with the control group (P <0.05), the number of lymphocyte in the experimental group was significantly higher than that of the control group on the 7th, 14th and 28th day after transplantation The level of IL-17 in the experimental group was significantly higher than that in the control group on the 28th day (P <0.05), and the incidence of obliterative bronchiolitis in the experimental group was 100.0% on the 28th day Higher than the control group (0.00%). Conclusion The model of chronic rejection after ectopic tracheal transplantation in rats was established and the pathological changes were analyzed. It was confirmed that the expression of IL-17 was positively correlated with the development of obliterative bronchiole fibrosis after ectopic tracheal transplantation.