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目的比较全国不同地区市售黄芪饮片的质量一致性,为其质量评价提供参考。方法采用HPLC指纹图谱法测定不同地区的26份市售黄芪饮片和对照药材的指纹图谱,色谱条件为:Angilent TC-C18(4.6 mm×250 mm,5μm)色谱柱;流动相为乙腈-0.05%磷酸水溶液;流速为1.0 ml/min;检测波长为203 nm。通过系统聚类分析、相似度计算等方法对结果进行分析比较。结果建立了黄芪饮片的HPLC指纹图谱分析方法;确定了25个共有特征峰;26份市售黄芪饮片被聚为两类,其相似度为0.731~0.975;26份市售黄芪饮片与对照药材蒙古黄芪指纹图谱的相似度结果为0.389~0.827,而与对照药材膜荚黄芪指纹图谱的相似度结果为0.195~0.425。结论不同地区的市售黄芪饮片的质量一致性欠佳,大多数饮片与蒙古黄芪比较一致。
Objective To compare the quality consistency of commercially available Astragalus Pieces in different parts of the country and provide a reference for its quality evaluation. Methods HPLC fingerprinting was used to determine the fingerprint of 26 samples of Astragalus membranaceus from different regions. The chromatographic conditions were as follows: Angilent TC-C18 (4.6 mm × 250 mm, 5 μm); mobile phase consisted of acetonitrile-0.05% Phosphoric acid solution; flow rate of 1.0 ml / min; detection wavelength of 203 nm. Through cluster analysis, similarity calculation and other methods to analyze and compare the results. Results The HPLC fingerprinting method of Radix Astragali decoction was established; 25 common characteristic peaks were identified; 26 samples of Radix Astragali were collected into two categories with similarities of 0.731-0.975; 26 samples of astragalus The similarity of the fingerprint of Radix Astragali was 0.389-0.827, while the similarity of the fingerprint of Astragalus membranaceus to the control was 0.195-0.425. Conclusion The quality consistency of the commercially available Astragalus Pieces in different regions is poor, most of the Pieces are consistent with Astragalus mongolica.