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目的研究檀香叶提取物的急性毒性和亚急性毒性,对其食用安全性进行评价。方法将昆明小鼠按性别体重随机分组,采用灌胃法,用檀香叶水提取物、95%乙醇提取物进行小鼠急性毒性实验,分别一次灌胃给药37 g/kg体重、38 g/kg体重,观察7 d,测量小鼠体重,脏器质量,并对肝脏和肾脏进行组织切片。用檀香叶醇提物进行小鼠亚急性毒性实验,1日1次灌胃给药25 g/kg体重,持续7 d,观察15 d,测量小鼠体重,脏器质量,并对肝脏和肾脏进行组织切片。结果 :(1)实验结束后,实验组和对照组小白鼠都无死亡;(2)宰杀的实验组小白鼠的各脏器无明显肉眼可见剖检病变;(3)实验组小白鼠的各脏器系数与对照组的差异不显著(P>0.1),组织病理检查未见异常。结论在本实验条件下,按急性毒性分类,檀香叶提取物属无毒级,檀香叶提取物未见明显的亚急性毒性。
Objective To study the acute toxicity and subacute toxicity of sandalwood leaf extract and evaluate the safety of its consumption. Methods Kunming mice were randomly divided into groups according to their sex and body weight. The mice were challenged with ganoderma lucidum leaf water extract and 95% ethanol extract by gavage. The mice were orally gavaged with 37 g / kg body weight and 38 g / kg body weight, observed for 7 days, measured body weight, organ quality, and the liver and kidney tissue sections. The mice were sub-acutely toxic with the extract of sandalwood leaves ethanol. The rats were administered intragastrically with 25 g / kg body weight once a day for 7 days for 15 days. The body weight and quality of the mice were measured, Kidney tissue sections. Results: (1) After the experiment, there was no death in the experimental group and control group of mice; (2) There was no obvious macroscopic necrosis in each organ in the experimental group; (3) There was no significant difference between the organ coefficient and the control group (P> 0.1), and no abnormality was found in the histopathological examination. Conclusion Under the experimental conditions, according to the classification of acute toxicity, sandalwood leaf extract is non-toxic grade, sandalwood leaf extract no significant subacute toxicity.