论文部分内容阅读
目的:探讨microRNA-34a(miR-34a)在肾癌细胞中的生物学作用及调控机制。方法:应用miR-34amimics在体外转染769P,786-O和Caki-1细胞;运用qRT-PCR检测miR-34a在三个细胞株的相对表达情况,以及转染后癌基因mRNA的表达情况;观察miR-34a对细胞生长的影响。结果:769P,786-O和Caki-1细胞中miR-34a在786-O中表达最低,769P次之,Caki-1表达最高;利用miR-34a mimics升高769P,786-O和Caki-1细胞miR-34a,发现三个细胞株多个癌基因mRNA表达不同程度的降低(P<0.05)及生长和集聚能力的降低。结论:miR-34a可能通过调控多个癌基因表达在肾癌中起抑癌作用。miR-34a mimics可抑制肾癌细胞的生长,因此miR-34a有可能作为肾癌基因治疗的新靶点。
Objective: To investigate the biological function and regulation of microRNA-34a (miR-34a) in renal cell carcinoma. Methods: The miR-34amimics were used to transfect 769P, 786-O and Caki-1 cells in vitro. The relative expression of miR-34a in three cell lines and the expression of oncogene mRNA were detected by qRT-PCR. To observe the impact of miR-34a on cell growth. Results: miR-34a was the lowest expression in 786-O, followed by 769P and Caki-1 in 769P, 786-O and Caki-1 cells. MiR-34a mimics increased 769P, 786-O and Caki-1 Cell miR-34a, found that three cell lines multiple oncogenes mRNA expression decreased to varying degrees (P <0.05) and growth and aggregation ability decreased. Conclusion: miR-34a may play a role in tumor suppressor in renal cancer by regulating multiple oncogenes. miR-34a mimics can inhibit the growth of renal cell carcinoma, so miR-34a may serve as a new target for gene therapy of renal cell carcinoma.