论文部分内容阅读
Objective:To investigate the role of 5-HT_7 receptor in the pathogenesis of irritable bowel syndrome(IBS).Methods:Rat model of D-IBS was established by intracolonic instillation of acetic acid and restraint stress;Rat model of C-IBS was established by stomach irrigated with 0-4℃cool water daily for 14 d.The content and distribution of 5-HT_7 receptor at the brain and bowel was examined by immunohistochemistry and the expression of 5-HT_7 receptor mRNA was detected by fluorescence quantitative RT-PCR(Real-time PCR).Results:Immunocytochemistry result showed the 5-HT_7 rceptor positive staining at hippocampus and hypothalamus of both C-IBS and D-IBS group was stronger than that of control group(P<0.01).The 5-HT_7R expression at ileum,proximate colon,distal colon of C-IBS group was significantly stronger than that of control group(P<0.05).Realtime-PCR analysis results showed the expression level of 5-HT_7 receptor at hippocampus and hypothalamus of both C-IBS and D-IBS group was increased than that of control group(P<0.05).At proximal and distal colon of C-IBS group,the 5-HT_7 receptor mRNA expression was increased compared with control group(P<0.05).Conclusion:The up-regulated expression of 5-HT_7 receptor at brain and colon may play an important role in the pathogenesis of C-IBS.
Objective: To investigate the role of 5-HT_7 receptor in the pathogenesis of irritable bowel syndrome (IBS). Methods: Rat model of D-IBS was established by intracolonic instillation of acetic acid and restraint stress; Rat model of C-IBS was established by stomach irrigated with 0-4 ° C cool water daily for 14 days. The content and distribution of 5-HT_7 receptor at the brain and bowel was examined by immunohistochemistry and the expression of 5-HT_7 receptor mRNA was detected by fluorescence quantitative RT-PCR (Real-time PCR) .Results: Immunocytochemistry result showed the 5-HT_7 rceptor positive staining at hippocampus and hypothalamus of both C-IBS and D-IBS group was stronger than that of control group (P <0.01). The 5-HT_7R expression at ileum, proximate colon, distal colon of C-IBS group was significantly stronger than that of control group (P <0.05) .Realtime-PCR analysis showed showed the expression level of 5-HT_7 receptor at hippocampus and hypothalamus of both C- IBS and D-IBS group was increase (P <0.05) .At proximal and distal colon of C-IBS group, the 5-HT_7 receptor mRNA expression was increased compared with control group (P <0.05) .Conclusion: The up-regulated expression of 5-HT_7 receptor at brain and colon may play an important role in the pathogenesis of C-IBS.