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目的:观察竹节人参提取物对急性低氧条件下大鼠脂质过氧化和微血管反应性的影响。方法:SD大鼠随机分为三组:对照组、模型组和竹节人参提取物组。对照组和模型组用生理盐水灌胃处理,竹节人参提取物组用竹节人参提取物灌胃处理30min后,急性低氧模型采用给予大鼠吸入含氧量为12.5%的氮氧混合气体,观察低氧10、15、20、25min时肠系膜微动脉血管口径、血流速度和25min时血中谷光甘肽过氧化物酶(GSH-px)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性和活性氧(ROS)含量的变化。结果:模型组与对照组比较,肠系膜微动脉血管口径增大(P<0.01,P<0.05),而血液流速却减慢(P<0.01,P<0.05),ROS明显增加(P<0.01),SOD、CAT和GSH-px活力明显下降(P<0.01);竹节人参提取物组能明显改善由于急性低氧而引起的上述多数指标(除血管口径外)的变化。结论:急性低氧可使肠系膜微动脉管径变大和血细胞流速减慢,同时体内抗氧化酶活性减弱;而竹节人参提取物能增加血细胞流速,提高抗氧化能力。
Objective: To observe the effects of ginseng extract on lipid peroxidation and microvascular reactivity in rats under acute hypoxia. Methods: SD rats were randomly divided into three groups: control group, model group and slubby ginseng extract group. The control group and the model group were treated with saline gavage. The ginseng extract group was treated with ginseng extract gavage for 30 minutes. The acute hypoxia model was administered to rats by inhalation of a mixture of nitrogen and oxygen containing 12.5% oxygen , The mesenteric arteriolar caliber, blood flow velocity and blood glutathione peroxidase (GSH-px), superoxide dismutase (SOD), peroxidation (CAT) activity and reactive oxygen species (ROS) content changes. Results: Compared with the control group, the diameter of mesenteric arterioles increased (P <0.01, P <0.05) and the blood flow velocity decreased (P <0.01, P <0.05) , SOD, CAT and GSH-px significantly decreased (P <0.01). The Scutellaria barbata extract group could significantly improve the changes of most of the above indicators except the caliber of the caliber caused by acute hypoxia. CONCLUSION: Acute hypoxia can increase the diameter of mesenteric arterioles and slow down the flow of blood cells. At the same time, the activity of antioxidant enzymes in vivo is weakened. However, the ginseng extract can increase the blood flow and increase the anti-oxidative capacity.