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Objective:To evaluate the antioxidant potential of different extract/fractions of Anthocephalus cadamba(A.cadamba)(Roxb.) Miq.(Rubiaceae) and study the tentative identification of their active constituents.Methods:The extract/fractions were screened for antioxidant activity using various in vitro assays viz.DPPH assay,ABTS assay,superoxide anion radical scavenging assay, reducing power assay and plasmid DNA nicking assay.Total phenolic content of extract/fractions was determined by colorimetric method.An ultra-performance LC-electrospray-quadrupoletime of flight mass spectrometry method was used to analyse the active constituents of extract/ fractions of A.cadamba.Results:The ethyl acetate fraction was found to be most active fraction in all the assays as compared to other extract/fractions.The IC_(50) value of ethyl acetate fraction (ETAC fraction) was 21.24μg/mL,1.12μg/mL,9.68μg/mL and S7.81μg/mL in DPPH assay, ABTS assay,reducing power assay and superoxide scavenging assay respectively.All the extract/ fractions also showed the potential to protect the plasmid DNA(pBR322) against the attack of hydroxyl radicals generated by Fenton’s reagent.The bioactive compounds were identified by UPLC-ESI-QTOF-MS,by comparing the mass andλ_(max) with literature values.Conclusions:The potential of the extract/fractions to scavenge different free radicals in different systems indicated that they may be useful therapeutic agents for treating radical-related pathologic damage.
Objective: To evaluate the antioxidant potential of different extract / fractions of Anthocephalus cadamba (A. cabada) (Roxb.) Miq. (Rubiaceae) and study the tentative identification of their active constituents. Methods: The extract / fractions were screened for antioxidant activity using various in vitro assays viz. DPPH assay, ABTS assay, superoxide anion radical scavenging assay, reducing power assay and plasmid DNA nicking assay. Total phenolic content of extract / fractions was determined by colorimetric method. An ultra-performance LC-electrospray-quadrupoletime of flight mass spectrometry method was used to analyze the active constituents of extract / fractions of A. cadamba. Results: The ethyl acetate fraction was found to be the most active fraction in all the assays as compared to other extracts / fractions. The IC 50 ) value of ethyl acetate fraction (ETAC fraction) was 21.24 μg / mL, 1.12 μg / mL, 9.68 μg / mL and S7.81 μg / mL in DPPH assay, ABTS assay, reducing power assay and superoxide scavenging assay respe ctively.All the extract / fractions also showed the potential to protect the plasmid DNA (pBR322) against the attack of hydroxyl radicals generated by Fenton’s reagent. bioactive compounds were identified by UPLC-ESI-QTOF-MS, by comparing the mass and λ_ ( max) with literature values. Conclusions: The potential of the extract / fractions to scavenge different free radicals in different systems indicates that they may be useful therapeutic agents for managing radical-related pathologic damage.