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以自组装方式,将多壁碳纳米管、聚酰胺-胺和辣根过氧化物酶修饰到玻碳电极表面,构建了H2O2生物传感器.研究了影响传感器工作性能的因素,确定了最佳分析条件,即pH值6.8的PBS缓冲溶液,工作电位为-0.15 V.在最佳工作条件下,电极对H2O2快速响应(响应时间<1 s),在9×10-7～1×10-4mol/L范围内,电极的电流响应值与H2O2浓度呈现良好的线性关系,线性回归方程为i(μA)=20.76 C(mmol/L)+0.223 0,R=0.999 0,检出限为5.0×10-7mol/L,电极的电流响应灵敏度为0.247 A mol-1.cm-2.电极应用于食用油脂中过氧化物测定,结果与碘量法一致.
The multi-walled carbon nanotubes, polyamidoamine and horseradish peroxidase were modified to the surface of glassy carbon electrode by self-assembly method, and the H2O2 biosensor was constructed.The factors influencing the working performance of the sensor were studied and the best analysis Conditions, pH 6.8 PBS buffer solution, the working potential of -0.15 V. Under the best working conditions, the electrode rapid response to H2O2 (response time <1 s), in the 9 × 10-7 ~ 1 × 10-4mol / L. The linear regression equation of the electrode showed a good linear relationship with the concentration of H2O2. The linear regression equation was: i (μA) = 20.76 C (mmol / L) +0.223 0, R = 0.999 0 with a detection limit of 5.0 × 10-7mol / L, the electrode current response sensitivity of 0.247 A mol-1.cm-2. The electrode used in the determination of peroxide in food fats, the results with the iodometric method.