论文部分内容阅读
目的 研究低温海水淹溺大鼠肺脏内皮素 (ET)及降钙素基因相关肽 (CGRP)含量变化规律 ,探讨二者对肺脏功能的影响。方法 将大鼠随机分为对照组和淹溺组。淹溺组大鼠分别置于 16～ 18℃海水中自由游动 ,于淹溺后 5 ,15 ,30 ,6 0 ,2 4 0 ,36 0 m in及死亡后测量肛温 ,自左心室取血 1ml行血气分析 ,处死动物取左肺计算肺干湿重比值 ,取右肺检测 ET和 CGRP含量。结果 海水淹溺后 ,不同时间组动物肛温、肺组织干湿重比值、p H、Pa O2 明显降低 ,其中肛温、p H、Pa O2 于 5 min时降至最低点 ,肺组织干湿重比值于 6 0 m in降至最低点 ;Pa CO2 则明显升高 ,于 5 m in组升至最高点 ;随后上述指标呈恢复趋势 ,然而除 p H于 2 4 0及 36 0 min时接近正常对照水平外 (P>0 .0 5 ) ,其余均仍未恢复到正常对照组水平 (P<0 .0 1)。 ET含量于 30 min和 CGRP含量于 15 m in分别降至最低点 [(15 .6 8± 6 .5 3) ng/ L及(34.4 7± 5 .5 8) ng/ L ],与正常对照组相比差异有显著性 (P<0 .0 1) ,随后呈上升趋势 ,前者至 2 4 0 m in高达 (5 8.92± 6 .39) ng/ L ,比正常对照组 (30 .72± 7.0 6 ) ng/ L明显增高 (P<0 .0 1) ,36 0 min时 (2 5 .6 9±4 .2 7) ng/ L恢复接近正常对照组水平 (P>0 .0 5 ) ;后者至 36 0 m in时 (49.6
Objective To investigate the changes of endothelin (ET) and calcitonin gene related peptide (CGRP) in the lungs of rats exposed to low temperature seawater and explore their effects on lung function. Methods The rats were randomly divided into control group and drowning group. Rats in the drowning group were respectively placed in seawater at 16-18 ° C for free swimming, and the rectal temperature was measured at 5, 15, 30, 60, 240, 360 and after death. Blood 1ml line blood gas analysis, executed animals were taken to calculate the left lung lung wet weight ratio, take the right lung test ET and CGRP content. Results After the sea water was drown, the rectal temperature and the ratio of dry weight to wet weight of lung tissue in different time groups were significantly lower than those in control group. The rectal temperature, p H and Pa O2 decreased to the lowest level at 5 min, Heavy ratio dropped to the lowest point at 6 0 m in; Pa CO2 increased significantly and rose to the highest point in 5 m in group; then the above indicators showed a trend of recovery, however, except when p H was close to 240 and 36 0 min Normal control level (P> 0.05), the rest are still not returned to the level of the normal control group (P <0.01). The content of ET decreased to the lowest level at 15 min after 30 min and CGRP content [(15.68 ± 6.35) ng / L and (34.4 7 ± 5.58) ng / L], respectively, (P <0.01), followed by an upward trend, the former to 240 m in up to (8.992 ± 6.39) ng / L, compared with the normal control group (30.72 ± (P <0.01) at 6 h, 7.0 6, and 25 ng / L at 36 0 min were close to that of the normal control group (P> 0.05) ; The latter to 36 0 m in (49.6