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目的建立SD鼠胰腺癌模型并探讨其癌变过程中病理形态学改变及胰腺上皮内瘤变(PanIN)的计数和分级。方法将二甲基苯并蒽(DMBA)置入胰实质内作为实验组(A组)及设立曲古霉素(TSA)干预组(B组),3～5个月内处死观察胰腺癌发生情况,苏木素-伊红(HE)染色镜下观察胰腺病理学变化及PanIN计数和分级。结果A组(实验组)发癌率为48.6%(18/37),其中胰腺导管癌17例和纤维肉瘤1例;B组发癌率为33.3%(12/36),其中胰腺导管癌11例和纤维肉瘤1例;A组肿块最大径明显大于B组(P<0.05);C组(对照组)胰腺及A、B组胰腺外主要脏器均未见肿瘤发生和无明显病理学改变。A组癌旁组织高级PanIN(HpanIN)数高于B组各时段,差异无统计学意义(P>0.05);A组非癌胰腺组织各时段HpanIN数高于B组,差异无统计学意义(P>0.05);A组和B组3个月鼠胰腺HpanlN数明显低于4个月鼠和5个月鼠,差异有统计学意义(P<0.05);C组仅见1个HpanIN,均明显低于A、B组鼠(P<0.01)。结论DMBA置入胰实质内可在短期获得较高的SD鼠胰腺癌发生率,TSA能抑制胰腺癌的发生和生长;HPanIN可能是胰腺导管癌发生过程中必不可少的重要病理形态学改变。
OBJECTIVE: To establish a rat model of SD rat pancreatic cancer and investigate its pathological changes and the counting and grading of pancreatic intraepithelial neoplasia (PanIN). Methods DMBA was placed in the parenchyma of the pancreas as experimental group (group A) and TSA intervention group (group B). The mice were sacrificed within 3 to 5 months to observe the occurrence of pancreatic cancer The pathological changes of pancreas and PanIN count and grading were observed under hematoxylin and eosin (HE) staining. Results The incidence of carcinogenesis in group A (experimental group) was 48.6% (18/37), including 17 cases of pancreatic ductal carcinoma and 1 case of fibrosarcoma. The incidence of carcinogenesis in group B was 33.3% (12/36), of which pancreatic ductal carcinoma 11 And fibrosarcoma in 1 case. The largest diameter of the tumor in group A was significantly larger than that in group B (P <0.05). No significant changes were found in the pancreatic tissues and the major organs outside of pancreas in groups A and B . The number of high grade PanIN (HpanIN) in adjacent tissues of group A was higher than that of group B (P> 0.05). The number of HpanIN in group A was higher than that in group B (P> 0.05), but the difference was not statistically significant P> 0.05). The HpanlN numbers of pancreas in 3 months mice in group A and group B were significantly lower than those in 4 months mouse and 5 months mouse (P <0.05); only 1 HpanIN was observed in group C Lower than A, B rats (P <0.01). Conclusion DMBA can be injected into the parenchyma of the pancreas to obtain a higher incidence of pancreatic cancer in SD rats in short term. TSA can inhibit the occurrence and growth of pancreatic cancer. HPanIN may be an important pathomorphological change in pancreatic ductal carcinomas.